• 文献标题:   Direct immune-detection of cortisol by chemiresistor graphene oxide sensor
  • 文献类型:   Article
  • 作  者:   KIM YH, LEE K, JUNG H, KANG HK, JO J, PARK IK, LEE HH
  • 作者关键词:   graphene oxide, cortisol, electrical sensor, chemiresistor sensor, adrenal gland, saliva
  • 出版物名称:   BIOSENSORS BIOELECTRONICS
  • ISSN:   0956-5663 EI 1873-4235
  • 通讯作者地址:   Myongji Univ
  • 被引频次:   9
  • DOI:   10.1016/j.bios.2017.07.017
  • 出版年:   2017

▎ 摘  要

In this study, a biosensor to detect a stress biomarker of cortisol using cortisol monoclonal antibody (c-Mab) covalently immobilized on reduced graphene oxide (rGO) channel as electrical sensing element was demonstrated. Highly specific immune-recognition between the c-Mab and the cortisol was identified and characterized on a basis of resistance change at the rGO channel based chemiresistor sensor achieving the limit of detection of 10 pg/mL (27.6 pM). In addition, cortisol concentrations of real human salivary sample and buffer solution of rat adrenal gland acute slices, which could secret the cortisol induced by adrenocorticotropic hormone (ACTH), were directly measured by the chemiresistor corresponding to the specific sensing of the cortisol. The rGO chemiresistor could selectively measure the cortisol levels in spite of diverse neuroendocrine's existence. The potential perspective of this study can be a protocol of new cortisol sensor development, which will be applicable to point-of-care testing (POCT) targeted for salivary cortisol, in vitro psychobiological study on cortisol induction, and implantable sensor chip in the future.