▎ 摘　　要

Chitin/graphene oxide (Ch/GO) composite aerogel beads were fabricated by a simple and environmentally friendly method for lipase immobilization. The morphology and specific surface area of these beads were investigated by scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET), respectively. The results revealed that the prepared beads showed three-dimensional porous structure, and GO was successfully bound to chitin with enhanced surface area and mechanical strength. Candida rugose lipase (CRL) was immobilized on Ch/GO aerogel beads under optimum conditions by adsorption and subsequent cross-linking with glutaraldehyde which is added in the presence of the unbound proteins by conjugation. The maximum immobilization capacity of Ch, Ch/GO-5, Ch/GO-10 were 129.9, 141.1, 151.9 mg/g(support). The immobilized lipases exhibited good temperature and thermal stability in comparison to free CRL. After recycling 5 times, the immobilized lipases remained more than 90% of their initial activity and the activity of Ch/GO-10 could remain 84.2% of free CRL. With these desired characteristics, the design of Ch/GO composite aerogel beads for lipase immobilization may have potential applications in various lipase-based industrial processes.