▎ 摘　　要

A room temperature ionic liquid (IL) I-butyl-3-methylimidazolium hexafluorophosphate functionalized graphene (GE) was prepared and a hydrogen peroxide (H2O2) biosensor was fabricated by immobilizing hemoglobin (Hb) into the IL-GE composite film. UV-visible and Fourier transform infrared spectra of the composite film indicated that Hb retained its native structure in the film. Electrochemical investigation of the biosensor showed a pair of well-defined, quasi-reversible redox peaks with E-pa = -0.209 V and E-pc = -0.302 V (vs. SCE) in pH 7.0 phosphate buffer solution at the scan rate of 100 mV/s. To the reduction of H2O2, the biosensor had a good linear range from 8.0 X 10(-7) 1.8 X 10(-4) mol/L with a detection limit of 3.0 X 10(-7) The apparent Michaelis-Menten constant (K-M(app)) was estimated to be 3.4 X 10(-5) mol/L.