▎ 摘　　要

Herein, an ultrasensitive and selective assay for antibiotic tetracycline (TC) was developed based on an aptamer and graphene oxide ( GO). The assay was designed based on the protection effect of the aptamer on GO due to aggregation. When the GO-based aptasensor was incubated with TC, TC could capture the TC aptamer from GO, leading to the aggregation of GO in a high salt solution. The increase in size and decrease in zeta-potential can be detected by adding TC in the assay. A linear relationship between the optical response and the logarithm concentration of TC was observed after centrifugation. The detection range was 0.002 to 20 ng mL(-1) and the detection limit was 0.001 ng mL(-1) (S/ N = 3). The recoveries of TC in honey samples (0.001 mg kg (1), 0.01 mg kg(1) and 0.1 mg kg (1)) were from 82.5% to 117%.