▎ 摘　　要

This work presents a facile method to availably synthesize the reduced graphene oxide (rGO), which utilizes the dopamine (DA) as the reducing agent. PDA/rGO was prepared by the simultaneous reduction of GO with dopamine hydrochloride and the self-polymerization of dopamine. The surface polydopamine (PDA) film was removed by immersing in a strong alkali solution (pH> 10) to obtain the pure rGO. Then 1-hydroxypyrene (1-OHP) was absorbed on the surface of rGO modified glassy carbon electrode (GCE) via pi-pi stacking interaction to fabricate a 1-OHP-rGo modified GCE (denoted as 1-OHP/rGO/GCE). The synergistic effect between the 1-OHP and rGO effectively reduced the oxidation over-potential of nicotinamide adenine dinucleotide (NADH), which realized the determination of NADH under relatively low potential (-0.06 V vs. SCE), which negatively shift 623 mV compared with that at the bare GCE. Further, the amperometric response of the modified electrode to NADH shows a linear concentration range of 100 mu M to 3800 mu M with the calculated limit of detection of 14.8 mu M at a signal-to-noise ratio of 3. We expect that this biosensor could be used for determination of NADH in biosamples.