▎ 摘　　要

The proposition of bio-material based soft hydrogel scaffold formation is one of the pivotal triggers to have the functionally suitable nano-bio composite. In this work, calf-thymus DNA (CT-DNA) and water-exfoliated 2D graphene oxide (GO) nanosheets have been executed towards the formation of nano and bio-material based flexible supramolecular hydrogel structure to achieve nano-bio functionality. Herein, calf thymus DNA and 2D GO nanosheets meditated a supramolecular hydrogel has been employed for the sun-ray directed synthesis of supramolecular hydrogel capped functional silver nanoparticles through a green synthetic protocol by reducing Ag(I) to Ag(0) without applying any added reducing agents. The CT-DNA and 2D GO nanosheets meditated a supramolecular hydrogel capped well-designed Ag-NPs (Ag-NPs@GO-DNA) have been employed for the production of smart ultrafine bacteria-mediated nano-bio composites. In this presentation, Gram + ve bacteria (Bacillus subtilis) and Gram -ve bacteria (Escherichia coli) have been chosen as the competent living ingredients for offering new class of Ag-NPs with considerable stability through exploiting the catalytic effects of the bacteria on Ag-NPs@GO-DNA. (C) 2021 Elsevier B.V. All rights reserved.