• 文献标题:   Graphene oxide/perylene-aniline electrochemiluminescence platform for protein detection based on molecule recognition
  • 文献类型:   Article
  • 作  者:   YIN P, ZHANG W, SHANG L, MA RN, JIA LP, WANG HS
  • 作者关键词:  
  • 出版物名称:   ANALYTICAL METHODS
  • ISSN:   1759-9660 EI 1759-9679
  • 通讯作者地址:  
  • 被引频次:   1
  • DOI:   10.1039/d1ay01588e EA OCT 2021
  • 出版年:   2021

▎ 摘  要

Most biosensors for folate receptor (FR) detection based on folic acid (FA) recognition usually contain FA-linked single-strand DNA (FA-ssDNA) and nuclease to promote sensitivity, which increases expenses and involves complicated assay processes. A few electrochemiluminescence (ECL) sensors that do not use FA-ssDNA and nuclease directly graft FA onto an ECL nanomaterial through covalent bonding for FR detection. In this study, we used FA-ssDNA to non-covalently graft FA onto pi-conjugated ECL nanomaterial graphene oxide (GO)/perylene-aniline for fabricating ultrasensitive FR sensors without nuclease. 3,4,9,10-Perylenetetracarboxylic dianhydride (PTCDA) and aniline (An) self-assembled into pi-conjugated nanorods, which were then loaded onto GO. This material was reported to produce 673 nm-dominated ECL with the co-reactant K2S2O8, and was used as an ECL platform. FA-modified Poly-dA-ssDNA (FA-Poly-dA-ssDNA) molecules, consisting of 20 bases, were attached to the surface of GO/PTCDA-An to capture FR. A significant decrease of ECL intensity was observed due to the steric hindrance of FR. The proposed sensors exhibited high detection sensitivity with a linear range from 1 fg mL(-1) to 1 ng mL(-1) and a detection limit of 0.636 fg mL(-1). The sensors also showed good potential in real sample detection. Without introducing nuclease and complicated chemical reactions, this work provides a new sensing strategy for protein detection based on molecular recognition, which is extremely important in clinical diagnosis.