• 文献标题:   A Graphene Oxide-Based Sensing Platform for The Label-free Assay of DNA Sequence and Exonuclease Activity via Long Range Resonance Energy Transfer
  • 文献类型:   Article
  • 作  者:   JIANG YX, TIAN JN, CHEN S, ZHAO YC, WANG Y, ZHAO SL
  • 作者关键词:   graphene oxide go, the long range resonance energy transfer lrret, exonuclease activity, dna
  • 出版物名称:   JOURNAL OF FLUORESCENCE
  • ISSN:   1053-0509 EI 1573-4994
  • 通讯作者地址:   Guangxi Normal Univ
  • 被引频次:   10
  • DOI:   10.1007/s10895-013-1189-7
  • 出版年:   2013

▎ 摘  要

Graphene oxide (GO) was introduced as an efficient quencher for label-free and sensitive detection of DNA. Probe DNA (pDNA) was mixed with ethidium bromide (EB) and graphene oxide (GO). The interaction between EB and GO led to the fluorescent quenching. Upon the recognition of the target, EB was intercalated into duplex DNA and kept away from GO, which significantly hindered the long range resonance energy transfer (LrRET) from EB to GO and, thus, increased the fluorescence of EB. The changes in fluorescent intensity produced a novel method for sensitivity, and specificity detection of the target. Based on the structure-switching of aptamers, this strategy could be conveniently extended for detection of other biomolecules, which had been demonstrated by the detection of exonuclease activity.