• 文献标题:   Cellular and molecular mechanistic insight into the DNA-damaging potential of few-layer graphene in human primary endothelial cells
  • 文献类型:   Article
  • 作  者:   SASIDHARAN A, SWAROOP S, CHANDRAN P, NAIR S, KOYAKUTTY M
  • 作者关键词:   fewlayer graphene, human primary cell, oxidative stres, microarray, dna damage
  • 出版物名称:   NANOMEDICINENANOTECHNOLOGY BIOLOGY MEDICINE
  • ISSN:   1549-9634 EI 1549-9642
  • 通讯作者地址:   Amrita Vishwa Vidyapeetham Univ
  • 被引频次:   19
  • DOI:   10.1016/j.nano.2016.01.014
  • 出版年:   2016

▎ 摘  要

Despite graphene being proposed for a multitude of biomedical applications, there is a dearth in the fundamental cellular and molecular level understanding of how few-layer graphene (FLG) interacts with human primary cells. Herein, using human primary umbilical vein endothelial cells as model of vascular transport, we investigated the basic mechanism underlying the biological behavior of graphene. Mechanistic toxicity studies using a battery of cell based assays revealed an organized oxidative stress paradigm involving cytosolic reactive oxygen stress, mitochondrial superoxide generation, lipid peroxidation, glutathione oxidation, mitochondrial membrane depolarization, enhanced calcium efflux, all leading to cell death by apoptosis/necrosis. We further investigated the effect of graphene interactions using cDNA microarray analysis and identified potential adverse effects by down regulating key genes involved in DNA damage response and repair mechanisms. Single cell gel electrophoresis assay/Comet assay confirmed the DNA damaging potential of graphene towards human primary cells. (C) 2016 Elsevier Inc. All rights reserved.