• 文献标题:   Preparation of Graphene/Gold Nanoparticles/Glucose Oxidase Flexible Electrode Based on Poly(ethylene terephthalate) Film and Its Application in Detection of Glucose
  • 文献类型:   Article
  • 作  者:   XU J, SUN W, HE LY, ZHANG Q, SU WJ
  • 作者关键词:   glucose, soft electrode, glucose oxidase, graphene, gold nanoparticle
  • 出版物名称:   CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
  • ISSN:   0253-3820 EI 1872-2040
  • 通讯作者地址:   Jimei Univ
  • 被引频次:   2
  • DOI:   10.11895/j.issn.0253-3820.170335
  • 出版年:   2017

▎ 摘  要

The graphene prepared by chemical vapor deposition onto copper foils was transferred onto a poly (ethylene terephthalate) (PET) soft substrate by the aid of poly. methyl methacrylate (PMMA). The soft G/ AuNPs/GOD composite electrode based on PET substrate was fabricated using a protocol in which the uniform distribution of Au nanoparticles (AuNPs) was firstly obtained by controlling the evaporation of gold sol on a graphene surface, then thioglycolic acid (TGA) was modified on the AuNPs through Au-S bond, and finally glucose oxidase (GOD) was immobilized on the surface of AuNPs through acylation reaction between TGA and the GOD. Glucose was detected in the linear range from 0. 05 to 10. 55 mmol/L with a linear correlation coefficient (r) of 0. 9955. The detection was performed in phosphate buffer solution (pH 7) at 25 degrees C with a working potential of 0. 6 V (vs. SCE electrode), and the detection limit was 1 mu mol/L (3 sigma). The G/AuNPs/ GOD flexible electrode based on PET substrate provided a new pathway to detect glucose in special environments using wearable equipment, which enlarged the applied field of glucose detection.