• 文献标题:   One-step fabrication of electrochemical biosensor based on DNA-modified three-dimensional reduced graphene oxide and chitosan nanocomposite for highly sensitive detection of Hg(II)
  • 文献类型:   Article
  • 作  者:   ZHANG ZH, FU XM, LI KZ, LIU RX, PENG DL, HE LH, WANG MH, ZHANG HZ, ZHOU LM
  • 作者关键词:   dnatemplated nanocomposite, detection of hg2+, electrochemical biosensor, chitosan, threedimensional reduced graphene oxide
  • 出版物名称:   SENSORS ACTUATORS BCHEMICAL
  • ISSN:   0925-4005
  • 通讯作者地址:   Zhengzhou Univ Light Ind
  • 被引频次:   40
  • DOI:   10.1016/j.snb.2015.11.091
  • 出版年:   2016

▎ 摘  要

A novel DNA-modified nanocomposite of three-dimensional reduced graphene oxide and chitosan particles (CS@3D-rGO@DNA) was prepared by one-step method and used as a new electrochemical bio sensing strategy to detect Hg2+ in drinking water. This biosensor was fabricated by the chemical coordination of T-Hg2+-T between the T-rich oligonucleotide strands contained in the as-prepared CS@3D-rGO@DNA nanocomposite and Hg2+, thereby varying the electrochemical signals. The developed electrochemical biosensor exhibited high sensitivity toward the determination of Hg2+ with a low detection limit of 0.016 nM, ranging from 0.1 nM to 10 nM. This novel bio sensor also showed high selectivity, good stability, and repeatability. Hence, this proposed CS@3D-rGO@DNA-based electrochemical strategy is feasible and may offer a simple, rapid, cost-effective, highly selective, and sensitive method for Hg2+ quantification. (C) 2015 Elsevier B.V. All rights reserved.