▎ 摘　　要

Graphene oxide (GO), used in a wide variety of applications, is increasingly being introduced into aquatic environments; this situation calls for research on GO toxicity to assess its environmental risks. In this study, the toxic effect of GO to E.coli was studied before and after its aggregation equilibrium in the synthetic surface waters (the soft water, moderately hard water, and hard water) to reveal the effects of GO aggregation and solution hardness. The cytotoxicity of GO increased with increasing solution hardness while decreased after GO aggregation. The 3 h 50% inhibitory concentration (IC50) values of dispersed GO in the soft water, moderately hard water, and hard water were 12.2 +/- 2.2, 8.5 +/- 1.5, and 4.0 +/- 1.0 mg/L, respectively. After 24 h shaking (aggregation equilibrium) in the synthetic surface waters, the dispersed GO aggregated and the 3 h IC50 values of GO aggregates in the three synthetic waters were 40.3 +/- 6.9, 15.9 +/- 2.2, and 7.5 +/- 1.5 mg/L, respectively. The dispersed GO sheets wrapped E. coli cells and cut the cell membrane, resulting in the disruption of cell membrane and the cell inactivation. With increasing water hardness, the heteroaggregation between GO sheets/aggregates and E. coli cells was enhanced, resulting in the increase of toxic effect. The GO aggregates could also entrap E.coli cells while exhibited limited effect on cell membrane disruption without sharp edges, thereby causing the lower toxic effect compared with the dispersed GO sheets. These outcomes shed new light on the assessment of ecological effects of GO.