▎ 摘　　要

The cholesterol level determination is a significant clinical diagnostic solution for heart and, thrombosis problems. In this work, we examined a novel non-enzymatic cholesterol biosensor using cholesterol oxidase (ChO(x)) enzyme immobilized on TiO2 nanoparticles influenced by reduced graphene oxide (rGO) -polypyrrole (PPy) (rGO-TiO2/PPy-GCE) nanocomposite was developed on a glassy carbon electrode (GCE) and the higher sensing response with lower detection limits were observed. The electrochemical properties of GCE modified PPy (PPy-GCE) were studied using CV (Cyclic Voltammetry) and DPV (Differential Pulse Voltammetry). The reported sensor exhibited piecewise linearity in the range of 0.1 mu M to 1 mu M and 1 mu M to 600 mu M with the sensing response of 61.665 and 0.1466 (2 mu A mu Mxcm) respectively. The detection limits of the sensor were found to be 32 nm. The results were repeatable and reproducible and this sensor can be applied to determine the cholesterol at real sample with satisfactory results.