▎ 摘　　要

Thin films have infinite potential uses in optics and other fields. They must be well suited to meet the requirements of its specific application in terms of thickness, optical properties, morphology and other characteristics. Herein, surface plasmon resonance (SPR) technique was employed to accurately determine the thickness and analyze the optical characteristics of graphene oxide/gold (GO/Au) multilayered structure in contact with the neurotransmitter dopamine (DA). Based on the theoretical fitting of the experimentally recorded SPR dips, n and k values of GO layer were 1.4400 and 0.0250, respectively, prior to DA injection. By increasing DA concentrations, SPR experimental curves were shifted to higher angles continuously. DA Binding to SPR chip which was confirmed by AFM and FTIR analysis has increased both n and thickness of GO film while decreased k. These results proved sensitivity enhancement of the modified chip towards DA and highlighted the technique's appropriate and sufficient potential to measure thickness and refractive index variations in real-time when 100 fM of DA was detected with sensitivity of 12.402 degrees /RIU.