▎ 摘　　要

A novel electrochemical immunosensor for sensitive detection of clenbuterol (CLB) is fabricated using glucose oxidase (GOD)-functionalized grahene oxide (GO) nanocomposites to label CLB. The immunosensor was constructed by layer-by-layer assembly colloidal prussian blue (PB), multiwalled carbon nanotubes (MWCNTs) and CLB antibodies (Abs) on a glassy carbon electrode (GCE). In this competitive immunoassay system, PB acts as the redox mediator to reduce H2O2 originated from the catalyst cycle of GOD. The high ratio of GOD to GO effectively amplified the signal for this competitive-type immunoassay. Under optimized conditions, the immunosensor shows a wide linear range from 0.5 to 1000 ng/mL with a low detection limit of 0.25 ng/mL. The dual signal amplification of GOD-functionalized GO nanocomposites as a label is promising to be applied to design other sensitive immunosenseors. (C) 2013 Elsevier B.V. All rights reserved.