▎ 摘　　要

To promote the development of molecular imprinting technique in the separation and analysis of oxycodone, molecularly imprinted polymer based on the magnetic graphene oxide and carbon dots nanoparticles (MIP@MGO/CDs NPs) was developed using ultrasonic-assisted dispersive solid-phase microextraction (UADSPME) and high performance liquid chromatography (HPLC). The morphology and structure characterization of synthesized nanoparticles are assessed by scanning electron microscope (SEM), Brunauer-Emmett-Teller analysis (BET), Fourier transform infrared spectroscopy (FTIR), vibrating sample magnetometer (VSM) and thermogravimetric analysis (TGA). The adsorption isotherms of MIPs followed the Langmuir model with the maximum adsorption capacity of 99 mg/g and imprinting factor of 3.39. Under optimized conditions, which achieved by central composite design under response surface methodology, the detection limit (S/N = 3) was obtained 0.80 ng/mL in the linear range of 1?2000 ng/mL. The average recoveries of oxycodone were calculated from 92.50 to 103.20% in human urine samples with RSD less than 3.65%.