▎ 摘　　要

Highly sensitive L-lysine enzyme electrodes were constructed by using poly(vinylferrocene)-multiwalled carbon nanotubes-gelatine (PVF/MWCNTs-GEL) and poly(vinylferrocene)-multiwalled carbon nanotubes-gelatine-graphene (PVF/MWCNTs-GEL/GR) composites as sensing interfaces and their performances were evaluated. Lysine oxidase (LO) was immobilized onto the composite modified glassy carbon electrodes (GCE) by crosslinking using glutaraldehyde and bovine serum albumin. Effects of pH value, enzyme loading, applied potential, electrode composition, and interfering substances on the amperometric response of the enzyme electrodes were discussed. The analytical characteristics of the enzyme electrodes were also investigated. The linear range, detection limit, and sensitivity of the LO/PVF/MWCNTs-GEL/GCE were 9.9 x 10(-7)-7.0 x 10(-4) M, 1.8 x 10(-7)M(S/N = 3), and 13.51 mu AmM-1 cm(-2), respectively. PVF/MWCNTs-GEL/GR-based L-lysine enzyme electrode showed a short response time (< 5 s) and a linear detection range from 9.9 x 10(-7) to 7.0 x 10(-4) M with good sensitivity of 17.8 mu A mM(-1) cm-2 and a low detection limit of 9.2 x 10(-8) M. The PVF/MWCNTs-GEL/GR composite-based L-lysine enzyme electrode exhibited about 1.3-fold higher sensitivity than its MWCNTs-based counterpart and its detection limit was superior to the MWCNTs-based one. In addition, enzyme electrodes were successfully applied to determine L-lysine in pharmaceutical sample and cheese.