▎ 摘　　要

The functionalized graphene nanosheets (PDDA-G) with poly(diallyldimethylammonium chloride) (PDDA) were synthesized and used to combine with room temperature ionic liquid (RTIL). The resulting RTIL/PDDA-G composite displayed an enhanced capability for the immobilization of hemoglobin to realize its direct electrochemistry. Moreover, the RTIL/PDDA-G based biosensor exhibited excellent electrocatalytic activity for the detection of nitrate with a wide linear range from 0.2 to 32.6 mu M and a low detection limit of 0.04 mu M at 3 sigma. This work opens a new way to functionalized graphene nanosheets with good biocompatibility and solubility in biosensors. (C) 2010 Elsevier B.V. All rights reserved.