▎ 摘　　要

The aim of this research was to create a non-enzymatic sensor for electrochemical glucose monitoring during the glycolytic process using functionalized graphene oxide decorated with silver nanoparticles modified glassy carbon electrode (Ag NPs/f-GOx/GCE). The Hummers method was used to synthesize GOx, which was then functionalized before being decorated with Ag NPs. The prepared GOx nanosheets oriented at different angles, rippled, and entangled with each other, and the Ag NPs in fcc structure were strongly and irregularly immobilized on GOx nanosheets in spherical form, according to morphological and structural studies using SEM and XRD analyses. The electrochemical studies using CV and amperometry techniques revealed that Ag NPs/f-GOx/GCE has a high stability and sensitivity, with sensitivity, detection limit, and linear range of 2.94 mu A/mu M, 0.001 mu M, and 0 to 28 mu M, respectively. When comparing the glucose sensing properties of Ag NPs/f-GOx/GCE to those of other non-enzymatic glucose sensors, it was discovered that Ag NPs/f-GOx/GCE had the lowest detection limit and the highest sensitivity values due to the synergetic effect of Ag NPs and f-GOx on Ag NPs/f-GOx/GCE, which have great structural characteristics and significant amounts of electroactive sites. The analysis of interference effect in the presence of potential interfering species during the glycolytic process revealed the prepared glucose sensor's high sensitivity and selectivity. The practical application of the proposed sensor to determine glucose in a fermented beer sample revealed that Ag NPs/f-GOx/GCE has the potential to be a reliable glucose determination sensor during glucose fermentation.