▎ 摘　　要

Protein surface imprinting produces materials capable of selective recognition and capture of proteins. Herein, a protein surface imprinted polymer on graphene oxide modified super-paramagnetic Fe3O4 nanoparticles is reported. The molecularly imprinted polymer was synthesized by ultrasound-assisted suspension polymerization, using ovalbumin as the template molecule, 3-aminophenylboronie acid as the functional monomer, and methylene-bis-acrylamide as the cross-linking agent. The nanoparticles were approximately 40 nanometers in size and super-paramagnetic. Moreover, these particles demonstrated considerably high adsorption capacity, fast adsorption kinetics, and selective binding affinities toward the template protein ovalbumin. The calibration curve of ovalbumin was linear from 5.0x10(-11) to 1.0x10(-10) molar. The limit of detection of ovalbumin was 2.0x10(-11)M. These results show that this super-paramagnetic material has potential for biological macromolecule separation and determination.