▎ 摘　　要

Owing to its unique surface properties, graphene can absorb environmental pollutants, thereby affecting their environmental behavior. Triphenyl phosphate (TPP) is a highly produced flame retardant. However, the toxicities of graphene and its combinations with contaminants remain largely unexplored. In this work, we investigated the toxicological effects of graphene and TPP to mussel Mytilus galloprovincialis. Results indicated that graphene could damage the digestive gland tissues, but no significant changes were found in the graphene + TPP co-exposure group. There was a significant decrease in the content of GSH and the activities of GST and CAT in the co-exposure group compared to that in graphene-exposed group. It seemed that the adsorption of TPP on graphene could inhibit the surface activity of graphene and thus reduced its tissue damage and oxidative stress in mussels. Expression levels of stress response (MyD88a), cytoskeleton (MHC1, PMyo and TMyo) and reproductive (CP450 and HSD) genes were up-regulated in the graphene-exposed group, but significantly down-regulated after combined exposure of graphene and TPP. Furthermore, PPI analysis proved that the interactions of HSP90AA1 with UNC45B and FKBP4/5/6/L contributed to the toxicity caused by the combined exposure. Because of the potential toxicity of graphene and TPP, government administrators should consider its risks prior to the widespread environmental exposure.