• 文献标题:   Screening and separation of alpha-amylase inhibitors from Solanum nigrum with amylase-functionalized magnetic graphene oxide combined with high-speed counter-current chromatography
  • 文献类型:   Article
  • 作  者:   CEN Y, XIAO AP, CHEN XQ, LIU LL
  • 作者关键词:   alphaamylase, highspeed countercurrent chromatography, magnetic graphene oxide, traditional chinese medicine
  • 出版物名称:   JOURNAL OF SEPARATION SCIENCE
  • ISSN:   1615-9306 EI 1615-9314
  • 通讯作者地址:   Chinese Acad Agr Sci
  • 被引频次:   1
  • DOI:   10.1002/jssc.201700333
  • 出版年:   2017

▎ 摘  要

A screening method using alpha-amylase-functionalized magnetic graphene oxide combined with high-speed counter-current chromatography was proposed and utilized to screen and separate alpha-amylase inhibitors from extract of Solanum nigrum. The alpha-amylase-functionalized magnetic graphene oxide was characterized and found to demonstrate satisfactory structure, magnetic response (24.5 emu/g), and reusability (retained 90% of initial activity after five cycles). The conditions for the screening with alpha-amylase functionalized magnetic graphene oxide were optimized and set at pH 7.0 and 25 degrees C. As a result, two potent flavonoid compounds, apigenin-7-O-glucuronide (1) and astragalin (2), were separated and collected through high-speed countercurrent chromatography and subjected to high-performance liquid chromatography analysis with purity higher than 90% (according to HPLC data), which were identified as alpha-amylase inhibitors. These results suggested that utilization of alpha-amylase functionalized magnetic graphene oxide in the rapid screening and isolation bioactive compounds from complex natural products is a feasible and environmentally friendly method.