▎ 摘　　要

In this manuscript, lucigenin (Luc) and Co2+ complex bifunctionalized graphene oxide hybrids (Co(Trp)(2)/Luc/GO) were synthesized via a facile and simple strategy by virtue of pi-pi stacking and electrostatic interaction. The as-prepared Co(Trp)(2)/Luc/GO hybrids exhibited good stability, water-solubility and excellent CL activity when reacted with H2O2, which was more than 30 times higher than Luc/GO. The CL mechanism between Co(Trp)(2)/Luc/GO and H2O2 has been proposed to be due to the fact that Co2+ could facilitate the formation of HO center dot, O-2(center dot-) and Co2+-HO2-, accelerating lucigenin CL reaction. Moreover, it was also found that ascorbic acid could directly react with Co(Trp)(2)/Luc/GO to generate CL emission in the presence of dissolved oxygen. By virtue of Co(Trp)(2)/Luc/GO as a platform, a sensitive, selective, reagent-less and disposable CL sensor for the detection of ascorbic acid was developed. Ascorbic acid could be determined in the range of 5.0 x 10(-7)-1.0 x 10(-3) M with a detection limit of 0.4 mu M. This work reveals that catalyst Co(Trp)(2) assembled on the surface of Luc/GO exhibits a unique catalytic effect on lucigenin CL reaction, which provides a new strategy for the synthesis of a lucigenin functionalized nanomaterial with high CL efficiency. The synthesized Co(Trp)(2)/Luc/GO may find more applications in the development of novel analytical methods.