• 文献标题:   Achieving direct electrochemistry of glucose oxidase by one step electrochemical reduction of graphene oxide and its use in glucose sensing
  • 文献类型:   Article
  • 作  者:   SHAMSIPUR M, TABRIZI MA
  • 作者关键词:   electrochemically reduced graphene oxide, surfactant, direct electrochemistry, glucose oxidase, glucose, biosensor
  • 出版物名称:   MATERIALS SCIENCE ENGINEERING CMATERIALS FOR BIOLOGICAL APPLICATIONS
  • ISSN:   0928-4931 EI 1873-0191
  • 通讯作者地址:   Razi Univ
  • 被引频次:   11
  • DOI:   10.1016/j.msec.2014.09.002
  • 出版年:   2014

▎ 摘  要

In this paper, the direct electrochemistry of glucose oxidase (GOD) was accomplished at a glassy carbon electrode modified with electrochemically reduced graphene oxide/sodium dodecyl sulfate (GCE/ERGO/SDS). A pair of reversible peaks is exhibited on GCE/ERGO/SDS/GOD by cyclic voltammetry. The peak-to-peak potential separation of immobilized GOD is 28 mV in 0.1 M phosphate buffer solution (pH 7.0) with a scan rate of 50 mV/s. The average surface coverage is 2.62 x 10-(10) mol cm(-2). The resulting biosensor exhibited a good response to glucose with linear range from 1 to 8 mM (R-2 = 0.9878), good reprodudbility and detection limit of 40.8 mu M. The results from the biosensor were similar (+/- 5%) to those obtained from the clinical analyzer. (C) 2014 Elsevier B.V. All rights reserved.