• 文献标题:   Sensitive electrochemical detection of hepatitis C virus subtype based on nucleotides assisted magnetic reduced graphene oxide-copper nano-composite
  • 文献类型:   Article
  • 作  者:   LI JW, LI YF, ZHAI XW, CAO Y, ZHAO J, TANG YG, HAN K
  • 作者关键词:   magnetic reduced graphene oxide, hepatitis c viru, electrochemical measurement, ultrasensitive detection
  • 出版物名称:   ELECTROCHEMISTRY COMMUNICATIONS
  • ISSN:   1388-2481 EI 1873-1902
  • 通讯作者地址:   Chinese Acad Sci
  • 被引频次:   3
  • DOI:   10.1016/j.elecom.2019.106601
  • 出版年:   2020

▎ 摘  要

In the paper, a DNA assisted magnetic reduced graphene oxide-copper nanocomposite (mrGO-CuNCs) is proposed. Copper ions are able to accelerate the oxidation of o-phenylenediamine. The electrochemical signal of the oxidized product, 2,3-diaminobenazine, are employed to characterize the HCV DNAs. The strategy is able to sensitively detect HCV DNA over a wide linear range within the range of 0.5-10 nM and has a detection limit of 405.0 pM. Critically, it could effectively discriminate the 1b and 6 k subtypes of HCV. This assay strategy has promising applications in the early diagnosis and blood screening of HCV.