• 文献标题:   A molecular beacon and graphene oxide-based fluorescent biosensor for Cu2+ detection
  • 文献类型:   Article
  • 作  者:   HUANG JH, ZHENG QB, KIM JK, LI ZG
  • 作者关键词:   biosensor, copper ion, molecular beacon, graphene oxide, fluorescence
  • 出版物名称:   BIOSENSORS BIOELECTRONICS
  • ISSN:   0956-5663
  • 通讯作者地址:   Hong Kong Univ Sci Technol
  • 被引频次:   49
  • DOI:   10.1016/j.bios.2012.12.056
  • 出版年:   2013

▎ 摘  要

In this work, we report a "turn-on" fluorescent strategy for the direct detection of Cu2+ in solutions using molecular beacons (MBs) and graphene oxide (GO). MBs are special single-stranded DNA and carry fluorescence sources. GO is a new nanomaterial having remarkable physical properties. In the sensing system, GO was used as an efficient fluorescence quencher upon the adsorption of MBs, which reduced the background signal and made the detection method highly sensitive. In the presence of Cu2+, the MBs were cut into short pieces and released by the GO, leading to fluorescence restoration. The detection limit of the sensing strategy was similar to 50 nM, which is sufficiently sensitive for practical applications. The sensing method also exhibited high selectivity in testing samples containing other metal ions. The application of the method for drinking water is demonstrated. (C) 2013 Elsevier B.V. All rights reserved.