▎ 摘　　要

By using graphene (RGO) as supporter, molecular recognition substance TBA as probe, thrombin as objective protein, electrochemical impedance spectroscopy (EIS) as determination technique, a method for the determination of proteins was developed. Because RGO can improve effective surface area of electrodes and accelerate electron transfer rate at electrode/electrolyte interface as well as TBA has the molecular recognition ability with the high specificity, this determination method possesses the high sensitivity and good specificity. In the linear range from 0.3 to 10 fmol/L for thrombin determination, the detection limit is 0.26 fmol/L. In this study, RGO was applied to the electrochemical aptasensor for the first time, demonstrating that RGO-modified electrode has the great potential for the application in the electrochemical aptasensor.