▎ 摘　　要

The purpose of this study was to investigate the effects of chitosan graphene oxide Codonopsis pilosula poly-saccharide (CS-GO-CPP) complex on the immune function of macrophage cells (RAW264.7). In this experiment, chitosan (CS) was combined with graphene oxide (GO) by electrostatic action to prepare CS-GO nanocomposites, and it was used as a carrier to load Codonopsis pilosula polysaccharide (CPP) onto CS-GO to prepare CS-GO-CPP. Using infrared spectroscopy detection, zeta potential detection, and thermogravimetric analysis, we conduct a preliminary analysis of the structure of CS-GO-CPP. Macrophages were employed to evaluate CS-GO-CPP immunomodulatory activity and the possible mechanism responsible for the activation of macrophages in vitro. The results showed that compared with CPP, CS-GO-CPP did not change the basic structure of poly-saccharide, and its thermal stability was improved. 0.78- 12.5 mu g.mL- 1 of CS-GO-CPP could significantly pro-mote the phagocytic activity of RAW264.7 cells (P < 0.05) and significantly increase NO content, IL-4 and IFN-gamma secretion, the expression of CD40, CD86, and F4/80 (P < 0.05). CS-GO-CPP might activate the NF-Kappa B signaling pathway and induce the nuclear translocation of NF-Kappa B p65. In conclusion, CS-GO-CPP has a capacity to activate RAW264.7 cells for an improvement of immunomodulation activities, which might be through NF-Kappa B signaling pathway.