▎ 摘　　要

This study describes the preparation and characterization of graphene oxide doped eggshell membrane (GO-ESM) as a novel electrochemical bioplatform for electroanalytical purposes. The GO-ESM bioplatform was prepared by incorporation of GO nano-sheets into the ESM via a facile sonication procedure. Field emission scanning electron microscopy and X-ray diffraction powder techniques were used to characterize the developed bioplatform. The electrochemistry of GO-ESM was investigated by decorating it on the surface of carbon ceramic electrode (CCE) by an O-ring. The GO-ESM platform was modified with Prussian blue (PB) via a facile dip-coating method. Then the resulted modified electrode (PB vertical bar GO-ESM vertical bar CCE) was used as a novel hydrogen peroxide electrochemical sensor. The fabricated electrode responds efficiently to H2O2 over the concentration range 125 nM-195 mu M with a detection limit of 31 nM (S/N = 3) and sensitivity 8.8 mu A mu M-1 cm(-2). The PB vertical bar GO-ESM vertical bar CCE has been successfully applied to determination of H2O2 content in spiked milk samples. Due to good stability, environmental friendly, cheapness, nontoxic, well behaved electrochemical properties, and biocompatibility, the fabricated bioplatform has the promising future for practical applications. (C) 2014 Elsevier B.V. All rights reserved.