▎ 摘　　要

The present article describes a miniaturized potentiometric D-dimer biosensor on graphene nanosheets with incorporated lipid films. The graphene electrode was used for the development of a very selective and sensitive immunosensor for the detection of D-dimer by immobilizing the mouse anti human D-dimer antibody on stabilized lipid films. The immunosensor responded for the wide range of D-dimer concentrations with fast response time of ca. 15 s. The presented potentiometric D-dimer biosensor is easy to construct and exhibits good reproducibility, reusability, selectivity, rapid response times, long shelf life and high sensitivity of ca. 59 mV/decade over the D-dimer logarithmic concentration range from 10(-6) mu g/L to 10(-3) mu g/L.