• 文献标题:   Size-Dependent Pulmonary Impact of Thin Graphene Oxide Sheets in Mice: Toward Safe-by-Design
  • 文献类型:   Article
  • 作  者:   RODRIGUES AF, NEWMAN L, JASIM D, MUKHERJEE SP, WANG J, VACCHI IA, MENARDMOYON C, BIANCO A, FADEEL B, KOSTARELOS K, BUSSY C
  • 作者关键词:   graphene oxide, inflammation, lung, macrophage, mice, rna sequencing
  • 出版物名称:   ADVANCED SCIENCE
  • ISSN:  
  • 通讯作者地址:   Univ Manchester
  • 被引频次:   2
  • DOI:   10.1002/advs.201903200 EA MAY 2020
  • 出版年:   2020

▎ 摘  要

Safety assessment of graphene-based materials (GBMs) including graphene oxide (GO) is essential for their safe use across many sectors of society. In particular, the link between specific material properties and biological effects needs to be further elucidated. Here, the effects of lateral dimensions of GO sheets in acute and chronic pulmonary responses after single intranasal instillation in mice are compared. Micrometer-sized GO induces stronger pulmonary inflammation than nanometer-sized GO, despite reduced translocation to the lungs. Genome-wide RNA sequencing also reveals distinct size-dependent effects of GO, in agreement with the histopathological results. Although large GO, but not the smallest GO, triggers the formation of granulomas that persists for up to 90 days, no pulmonary fibrosis is observed. These latter results can be partly explained by Raman imaging, which evidences the progressive biotransformation of GO into less graphitic structures. The findings demonstrate that lateral dimensions play a fundamental role in the pulmonary response to GO, and suggest that airborne exposure to micrometer-sized GO should be avoided in the production plant or applications, where aerosolized dispersions are likely to occur. These results are important toward the implementation of a safer-by-design approach for GBM products and applications, for the benefit of workers and end-users.