▎ 摘　　要

The construction and biofunctionalization of graphene-based conjugated polymer nanocomposite that included reduced graphene oxide-poly DTP-NH2 hybrid-modified glassy carbon electrode (GCE) for the electrochemical H2O2 sensing were accomplished in this work. For the construction of the hydrogen peroxide bioassay, as an initial step of fabrication of the nanobiosensor, a novel conductive polymer structure was synthesized by the Suzuki-Miyaura coupling reaction and structural characterization was carried out by H-1-NMR and Mass spectroscopy techniques. Then, graphene oxide was chemically synthesized according to Hummers' method to expand the effective surface area to enhance the interaction between enzymes and conjugated polymers. This synthesized structure was assembled by an electrochemically polymerized conductive polymer with graphene oxide on a GCE. The modified glassy electrode surface was obtained by covalent bonding of the electrochemically polymerized DTP-NH2 with graphene oxide and coated on each well as biomimetic material. The structure and sensing properties of the nanosensing hybrid structure were investigated using UV-vis, AFM, SEM, and XRD. Thanks to the fast electron transfer at the poly(DTP-NH2)/rGO nanocomposite electrode interface, the developed biosensor exhibits a fast and linear amperometric response upon H2O2. Finally, a biosensor that is more selective and sensitive to H2O2 was developed. Also, the Poly(DTP-NH2)/rGO nanocomposite based Hydrogen Peroxide Nano sensor displayed excellent selectivity as well as good reproducibility and stability. (c) 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 48538.