▎ 摘　　要

Graphene oxide (GO), one of the water-soluble derivatives of graphene, could initiate strong chemiluminescence (CL) emission of luminol in the absence of any oxidant, and then the CL intensity was inhibited by puerarin (PUE), a main component in the traditional Chinese medicine Gegen. Based on this, a novel CL method was established for the determination of PUE. This method showed a linear relationship between the CL signal and the logarithm of PUE concentration in the range 0.01-6.00 mu M. The limit of detection was 2.83 nM and the relative standard deviation (RSD) was 1.94% for 11 determinations of 0.4 mu M PUE. This method was successfully used for the determination of PUE in Gegen and human urine samples. The possible CL reaction mechanism was investigated by UV-vis, fluorescence, and CL spectra, as well as by some chemical experiments.