▎ 摘　　要

The large-scale applications of lysozyme in the pharmaceutical industry and food industry require more efficient and cost-effective techniques for its separation/purification. In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions. Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance. This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA. Batch adsorption experiments found that the lysozyme adsorption capacity of Ca-SA/GO can reach 278.28 mg g(-1) and it can be regenerated and reused at least 4 times. Moreover, in column adsorption, the Ca-SA/GO showed excellent dynamic adsorption property. With good stability, adsorption capacity, and regeneration ability, the Ca-SA/GO could be a promising adsorbent for lysozyme from aqueous solutions.