• 文献标题:   Screening of the antioxidant properties of olive (Olea europaea) leaf extract by titanium based reduced graphene oxide electrode
  • 文献类型:   Article
  • 作  者:   YAZAR S, KURTULBAS E, ORTABOY S, ATUN G, SAHIN S
  • 作者关键词:   olea europaea, food analysi, cyclic voltammetry, square wave voltammetry, optimization
  • 出版物名称:   KOREAN JOURNAL OF CHEMICAL ENGINEERING
  • ISSN:   0256-1115 EI 1975-7220
  • 通讯作者地址:   Istanbul Univ Cerrahpasa
  • 被引频次:   2
  • DOI:   10.1007/s11814-019-0288-9
  • 出版年:   2019

▎ 摘  要

Olive leaves were extracted with homogenizer-assisted extraction (HAE). Box-Behnken (BBN) design was employed through response surface approach (RSA) to achieve the optimum conditions. Bioactivity of the extract was assessed by its oleuropein, total biophenol (TBC) and total flavonoid (TFC) content along with its antioxidant activity determined by DPPH and CUPRAC assays. A new nanocomposite was developed using reduced graphene oxide (rGO) modified with TiOx (Ti-rGO) for trace amount determination of oleuropein in olive leaf extract. Structural characterization of the electrode was clarified using atomic force microscopy (AFM) and diffuse reflectance infrared Fourier transform spectroscopy (DRIFT) techniques. Cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques were performed to investigate electrochemical behavior of oleuropein using three electrode configurations. Results of CV and SWV showed that quasi-reversible reaction occurred on electrode/electrolyte interface and a linear concentration range of 5-30 nM was obtained with a detection limit of 0.57 nM for oleuropein.