▎ 摘　　要

Background: Nanotechnology is used in stem cell culture as well as in vivo delivery and tracking of stem cells. Graphene oxide (GO) is a carbon based nanomaterial and it has large surface area as well as good biocompatibility and heteroatoms doped GO exploit its properties. Hybrid GO ( hGO) nano structures biocompatibility is depends on its size, dose and exposure time as well as in vitro cell models and hence, need thorough cytotoxicity studies in different species in vitro cell models. Methods: Caprine Wharton's jelly derived mesenchymal stem cells (WJ-MSCs) were isolated, characterized and dose dependent (100, 50, 25, 10 and 0 mu g /ml) in vitro cytotoxicity of three different hGO nano structures (phosphorus doped graphene oxide titanium oxide tubes, rods and sheets) were analysed in caprine WJ-MSCs by studying cell cytotoxicity assays. Result: All three hGO nano structures were damaged cell morphology at 100 and 50 mu g /ml doses, however, morphologically more good cells were observed in hGO tubes treated group than hGO rods and hGO sheets at 25 and 10 mu g/ml doses as compared to control. Cell viability percentage was significantly (P<0.01) decreased at dose 100 mu g/ml and it was significantly ( P< 0.01) increased at 25 mu g/ml dose as compared to 50, 10 and 0 mu g/ml doses. But, hGO tubes significantly (P<0.01) increased cell viability % as compared to hGO rods and hGO sheets. Cell population doubling time (PDT) was not altered significantly by all hGO nano structures, but 100 and 50 mu g/ml doses significantly (P<0.01) increased cell PDT as compared to 25, 10 and 0 mu g/ml doses. All hGO nano structures were non significantly altered growth curve, however, all hGO nano structures at 25 mu g /ml dose altered (inclined) shape of growth curve, while 100 and 50 mu g /ml doses significantly declined growth curve shape as compared to 10 and 0 mu g /ml doses. Cell proliferation % was significantly (P<0.01) increased at 25 and 10 mu g/ml doses, while, it was significantly (P<0.01) decreased at 100 mu g /ml dose as compared to 50 and 0 mu g /ml. However, there was no significance difference was observed in cell proliferation % in groups treated by different hGO nanostructures. In last, it was concluded as, hGO nano structures cytotoxicity was dose dependent and hGO nano tubes were least cytotoxic in caprine WJ-MSCs.