▎ 摘　　要

In this work, PtCu bimetallic nanoparticle was deposited on poly (styrene sulfonate) (PSS) functionalized graphene (Gr) to form a nanocomposite PtCu/PSS-Gr and its enzyme-like activity was investigated. Benefiting from the synergistic effect from Pt and Cu monometal as well as the superior properties of PSS-Gr, such as large surface area, good dispersity, strong adsorption of substrate and additional peroxidase-like activity, the PtCu/PSS-Gr nanocomposite was demonstrated as an excellent peroxidase mimic to catalyze the oxidation of 3,3'5,5'-tetramethylbenzidine (TMB) in the presence of H2O2. Combined with molecularly imprinted polymer (MIP), a new colorimetric approach for puerarin detection was proposed with the linear range of 2 x 10(-5) 6 x 10(-4) mol L-1 and LOD of 1 x 10(-5) mol L-1. The combination of MIP with PtCu/PSS-Gr nanocomposite not only endowed the determination of puerarin with high selectivity, but also realized the detection of small molecules which are neither substrate of the nanozyme nor substances with strong oxidizing or reducing activity by using peroxidase-like catalytic activity of nanozyme, expanding the application of nanozyme.