• 文献标题:   Label-free detection of alanine aminotransferase using a graphene field-effect biosensor
  • 文献类型:   Article
  • 作  者:   HER JL, PAN TM, LIN WY, WANG KS, LI LJ
  • 作者关键词:   graphene fieldeffect transistor gfet, alanine aminotransferase alt, lalanine, alphaketoglutarate
  • 出版物名称:   SENSORS ACTUATORS BCHEMICAL
  • ISSN:   0925-4005
  • 通讯作者地址:   Chang Gung Univ
  • 被引频次:   13
  • DOI:   10.1016/j.snb.2013.03.026
  • 出版年:   2013

▎ 摘  要

In this paper, we developed a low operation voltage and a single reaction step of a graphene field-effect transistor (GFET) biosensor device to detect alanine aminotransferase (ALT). The device is fabricated using large-area graphene thin films by means of chemical vapor deposition. The GFET device operated at 0.1 V drain voltage and -0.1 to 0.3 V sweep gate voltage exhibited a high pH sensitivity of 23.12 mV/pH, a low hysteresis voltage of 1.2 mV, and a small drift rate of 4.74 mV/h. In this research, the hybrid configuration of L-alanine and alpha-ketoglutarate immobilized on the graphene membrane was proved to be able to detect ALT with good linearity (R-2 = 0.99) in the ALT concentration range of 10-100 U/L. (C) 2013 Elsevier B.V. All rights reserved.