▎ 摘　　要

Hepatitis B virus core antigen (HBcAg) is the major structural protein of hepatitis B virus (HBV). The presence of anti-HBcAg antibody in a blood serum indicates that a person has been exposed to HBV. This study demonstrated that the immobilization of HBcAg onto the gold nanoparticles-decorated reduced graphene oxide (rGO-enAuNP5) nanocomposite could be used as an antigen-functionalized surface to sense the presence of antiHBcAg. The modified rGO-en-AuNPs/HBcAg was then allowed to undergo impedimetric detection of antiHBcAg with anti-estradiol antibody and bovine serum albumin as the interferences. Upon successful detection of anti-HBcAg in spiked buffer samples, impedimetric detection of the antibody was then further carried out in spiked human serum samples. The electrochemical response showed a linear relationship between electron transfer resistance and the concentration of anti-HBcAg ranging from 3.91 ng mL(-1) to 125.00 ng mL(-1) with lowest limit of detection (LOD) of 3.80 ng inL(-1) at 3 sigma m(-1). This established method exhibits potential as a fast and convenient way to detect anti-HBcAg. (C) 2018 Elsevier B.V. All rights reserved.