▎ 摘　　要

Purification of rhamnolipids (RLs) from its fermentation broth has been thought as the main limitation for application of RLs at large-scale. In this work, cationic graphene oxide (C-GO) has been prepared by 3-chloro-2-hydroxypropyl trimethyl ammonium chloride and graphene oxide for adsorbing rhamnolipids (RLs) from the fermentation broth. The adsorption capacity of C-GO for RLs was influenced by both the preparing conditions and the adsorption process. After optimizing the preparing parameters, the Zeta potential of the prepared C-GO was as high as + 23.01, and its corresponding adsorption capacity for RLs reached 2.0 g/g C-GO. The maximum adsorption capacity of C-GO reached within 2 min when the initial pH of RLs' fermentation broth was 4.0, the initial concentration of RLs was 16 g/L and the concentration of C-GO added was 4 g/L. Ethanol (99.5%) could recover 90% of RLs adsorbed on the surface of C-GO. The chromatographic purity of recovered RLs was above 90%. Based on the above results, C-GO, with quick, high specific adsorption and easy desorption property for RLs, has great potential in application of RLs' separation.