▎ 摘　　要

NOVELTY - Preparation of electrochemical immunosensor involves coating the 10 mu L 1 mg/ml human immunoglobulin G solution on activated-3,5-dibromosalicylaldehyde Schiff base nickel complex-graphene oxide-modified gold electrode, storing the obtained electrode in refrigerator for 4 degrees C, coating 10 mu L 1 mg/ml bovine serum albumin solution on the surface of the resultant electrode, blocking the active sites on the electrode surface, performing infrared drying and storing the electrode in refrigerator for 4 degrees C. USE - Preparation of electrochemical immunosensor (claimed). ADVANTAGE - The method efficiently provides electrochemical immunosensor, by a simple process. DETAILED DESCRIPTION - Preparation of electrochemical immunosensor involves dissolving 10 mg graphene oxide and 0.05 mmol 3,5-dibromosalicylaldehyde Schiff base nickel complex in N,N-dimethylformamide, ultrasonically dispersing for 2 hours, obtaining 3,5-dibromosalicylaldehyde Schiff base nickel complex-graphene oxide suspension, placing gold electrode in the obtained 3,5-dibromosalicylaldehyde Schiff base nickel complex-graphene oxide suspension, performing infrared drying, obtaining 3,5-dibromosalicylaldehyde Schiff base nickel complex-graphene oxide-modified gold electrode, horizontally placing the obtained 3,5-dibromosalicylaldehyde Schiff base nickel complex-graphene oxide-modified gold electrode in mixed solution obtained by mixing 10 mu L 1 mg/ml 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide or 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and N-hydroxysuccinimide for 2 hours, activating, obtaining activated-3,5-dibromosalicylaldehyde Schiff base nickel complex-graphene oxide-modified gold electrode, storing the obtained electrode in refrigerator for 4 degrees C, coating the 10 mu L 1 mg/ml human immunoglobulin G solution on the obtained activated-3,5-dibromosalicylaldehyde Schiff base nickel complex-graphene oxide-modified gold electrode, storing the obtained electrode in refrigerator for 4 degrees C, coating 10 mu L 1 mg/ml bovine serum albumin solution on the surface of the resultant electrode, blocking the active sites on the electrode surface, performing infrared drying, and storing the electrode in refrigerator for 4 degrees C.