▎ 摘　　要

NOVELTY - Manufacturing protein sensor, comprises: providing a hydrophobic material, where the hydrophobic material comprises a surface; performing an atmospheric pressure plasma process to form a hydrophilic functional group on the surface of the hydrophobic material; immobilizing a first antibody on the surface of the hydrophobic material by the hydrophilic functional group; preparing a mixed solution, where the mixed solution comprises a second antibody and an analyte, and the second antibody binds to the analyte; and reacting the mixed solution with the first antibody immobilized on the surface of the hydrophobic material to bind the first antibody to the analyte. The hydrophobic material comprises graphite, graphene, or gold sheet. The hydrophilic functional group comprises carboxyl group, and/or hydroxyl group. The source of the analyte comprises a cell extract or serum. The analyte is a tumor marker CA125. USE - The method is useful for manufacturing protein sensor. ADVANTAGE - The method: provides sensor that to identify and bind to analyte that binds to second antibody; has first antibody may be directly bonded to hydrophilic functional group in chemical bonding manner, so that first antibody may be immobilized on hydrophobic material and may not be easy to fall off; the first antibody and second antibody may simultaneously identify and bind to different positions on analyte, so that analyte is located between first antibody and second antibody; and provides protein sensor to identify types and quantities of proteins and having relatively low detection limit and relatively good sensitivity.