▎ 摘　　要

NOVELTY - Apparatus (A1) comprises: an exhaled breath condensate (EBC) collector (102) for converting breath vapor received from the lungs and airways of a test subject into an EBC fluid biosample; a biomarker concentrator for concentrating a target biomarker portion in the fluid biosample to form a concentrated fluid biosample; and a biomarker testing unit for receiving the concentrated fluid biosample and testing the concentrated fluid biosample for a target biomarker. USE - The apparatus is useful: for assembling an array of applied-field-reactive capture molecule conjugates; in an EBC collector for converting breath vapor received from the lungs and airways of the test subject into a fluid biosample; for forming a condensate collector having fluid conductor channels on a substrate for guiding a flow of fluid towards a desired direction; in a capture molecule conjugate for detecting a target analyte; for concentrating a target analyte in an EBC sample; for testing EBC for a target biomarker; in an electronic biosensor; in a sensor for detecting target molecules in a fluid sample; in a lateral flow assay, a room scale biosensor, a FET sensor circuit and a packaged biosensor semiconductor device (all claimed); for detecting a biomarker contained in exhaled breath of a test subject; for detecting a biomarker of a biological agent e.g. a coronavirus, lung cancer, tuberculosis, asthma, and other respiratory ailments and conditions, and/or blood borne biomarkers and other biomarkers present in the exhaled breath of a test subject; and for detecting biometric parameters for treating and monitoring of physiological conditions in humans and animals. No biological data given. ADVANTAGE - The apparatus: utilizes aptamers that exhibit many benefits as recognition elements when compared to traditional antibodies due their small size, enhanced chemical stability and low cost of production; has the potential of a much higher sensitivity, and provides a direct-to-electrical signal to enable e.g. easy wireless connectivity; and enables the simultaneous detection of a number of biomarkers, and increases the accuracy of test by using multiple biosensors. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: assembling (M1) an array of applied-field-reactive capture molecule conjugates, comprising providing dissolvable adhesive film, providing a carrier fluid that is a non-solvent for the dissolvable adhesive film, where the carrier fluid has randomly dispersed applied-field-reactive capture molecule conjugates, applying an aligning field to the carrier fluid for assembling the applied-field-reactive capture molecule conjugates onto the dissolvable adhesive film, and evaporating the carrier fluid leaving the assembled applied-field-reactive capture molecule conjugates fixed on the dissolvable adhesive film; an EBC collector for converting breath vapor received from the lungs and airways of the test subject into a fluid biosample, including a condensate-forming surface and a thermal mass in thermal connection with the condensate-forming surface, and a fluid transfer (104) system for transferring the EBC to at least one of a testing unit and an EBC containment vessel; forming (M2) a condensate collector having fluid conductor channels on a substrate for guiding a flow of fluid towards a desired direction, comprising providing the substrate having a surface having a relatively lower energy surface property, and forming a textured structure forming fluid conductor channels on the surface having a relatively higher energy surface property for guiding a flow of fluid towards a desired direction; detecting (M3) a target analyte; a capture molecule conjugate for detecting a target analyte, comprising an applied-field-reactive capture molecule conjugate having at least one applied-field-responsive end and at least one capture molecule end, where each capture molecule end binds to and captures the target analyte, and the binding of each capture molecule end to the target analyte increases an electrical charge difference between the applied-field-responsive end and the capture molecule end; concentrating (M4) a target analyte in an EBC sample; an electronic (B1) biosensor comprising a substrate having a water absorbing property provided by at least one of a selectively permeable membrane, a super absorbent polymer (SAP), a microfluidic material, and a wick, at least two electrodes formed on a top surface of the substrate defining a gap there between, and a functionalized detector provided in the gap and comprising an electron transport material and a capture molecule, where a target molecule captured by the capture molecule causes a change in at least one of a polarity and conductivity of the electron transport material; a sensor (S1) for detecting target molecules in a fluid sample; detecting (M5) a target molecule from a fluid sample; a sensor (S2); a lateral flow assay comprising a sample pad provided at a first stage of the lateral flow assay, a selectively permeable membrane provided adjacent to the sample pad for allowing a portion of a fluid sample to pass through the sample pad and for blocking at least some target molecules to accumulate target molecules in another portion of the fluid sample that does not pass through the selectively permeable membrane, a fluid absorber provided in fluid communication with the selectively permeable membrane to absorb the portion of the fluid sample that passes through the selectively permeable membrane, and a dissolvable fluid dam for holding the another portion of the fluid sample having accumulated target molecules from flowing to a next stage of the lateral flow assay; a room (B2) scale biosensor comprising an intake for taking in ambient air, a condenser for cooling the ambient air to condense moisture in the ambient air to a condensate containing water and at least one target molecule, and a condensate testing system for testing the condensate for the target molecule, where the condensate testing system includes a biosensor comprising at least one graphene (g-FET) biosensor having a detection interface comprising a graphene layer functionalized with capture molecules, and the capture molecules are smaller than the Debye screening length.; a FET sensor circuit (C1) for detecting target molecules in a fluid sample; and a packaged biosensor semiconductor device (D1). DESCRIPTION OF DRAWING(S) - The figure shows a face mask and schematic view of an exhaled breath condensate (EBC) collector, fluid transfer system, biosensor testing system and near field communication (NFC) antenna and signal condition circuit all disposed within the confined volume of the inside of the face mask. 102EBC collector 104Fluid transfer 106NFC antenna 108Face mask