▎ 摘　　要

NOVELTY - An electrochemiluminescence aptamer sensor preparing method involves preparing magnetic material graphene oxide solution by taking flask, adding graphene oxide, cooling and washing to obtain magnetic material graphene oxide solution. The graphene oxide solution is added with coupling reagent, incubated, added with electrochemiluminescent precursor and aptamers to obtain multifunctional graphene oxide material solution aptamers and electrochemiluminescence body, followed by washing magnetic glassy carbon electrode, polishing, applying graphene oxide material to obtain the finished product. USE - Method for preparing electrochemiluminescence aptamer sensor for detecting tumor marker MUC1 (claimed). ADVANTAGE - The method enables preparing electrochemiluminescence aptamer sensor with high specificity, and can detects the tumor marker MUC1 in simple, rapid, sensitive and accurate manner. DETAILED DESCRIPTION - An electrochemiluminescence aptamer sensor preparing method involves taking clean three-necked flask, adding 30-50 ml 1 mg/ml graphene oxide, treating ultrasonically for 1 hour, adding 20-30 ml 0.05-0.07 mol/l ferrous chloride solution and 20-30 ml 0.10-0.15 mol/l high chloride iron solution under nitrogen atmosphere protection, stirring at 70-90 degrees C, adding 10-15 ml 20-30% ammonia solution, treating for 3-5 hours, cooling and washing to obtain magnetic material graphene oxide solution, and storing at 4 degrees C. The magnetic material graphene oxide solution is placed in glass vial, treated ultrasonically for 1 hour, added with 200-400 mu l coupling reagent, mixed, added with dilute hydrochloric acid, incubated for 1 hour, washed with water, added with 30-50 mu l 0.001-0.1 mol/l electrochemiluminescent precursor solution and 30-50 mu l 10-20 mu mol/l aptamers solution, mixed, added with sodium hydroxide solution, incubated for 4 hours, washed, add with 1-2% bovine serum albumin, immersed at 4 degrees C for 1 hour, washed with secondary water to obtain multifunctional graphene oxide material solution aptamers and electrochemiluminescence body, and stored at 4 degrees C, followed by washing magnetic glassy carbon electrode, polishing with aluminum oxide polishing paste, ethanol and water, ultrasonicating for 2 minutes under dry nitrogen, applying multifunctional graphene oxide material solution on the glassy carbon electrode surface and attaching the electrode surface to obtain the finished product. An INDEPENDENT CLAIM is also included for a method for detecting tumor marker MUC1, which involves immersing electrochemiluminescence aptamer sensor in different concentrations of tumor marker MUC1 solution, incubating at 37 degrees C for 1 hour, washing using water as the working electrode, taking molybdenum wire electrode as an electrode, silver/silver chloride molybdenum wire electrode as an electrode, placing the three-electrode system in a buffer solution, starting the electrochemical reaction, measuring electrochemiluminescent intensity, followed by calculating different concentrations of tumor marker MUC1 solution corresponding electrochemiluminescent intensity values, establishing a quantitative relationship between electrochemiluminescent intensity value and tumor marker MUC1 concentration and detecting the tumor marker MUC1 according the quantitative relationship.