▎ 摘　　要

NOVELTY - A multi-walled carbon nanotube functionalized graphene composite material is prepared by adding sodium 1,3,6,8-pyrenetetrasulfonate solution to reduced graphene oxide dispersion, stirring, centrifuging and vacuum-drying to obtain functionalized graphene, preparing graphene suspension using functionalized graphene, mixing the graphene suspension with sodium 1,3,6,8-pyrenetetrasulfonate solution to obtain reaction solution, mixing reaction solution and multi-walled carbon nanotube dispersion, centrifuging, washing and vacuum-drying. USE - Multi-walled carbon nanotube functionalized graphene composite material is used as adsorbent for determination of beta -2 receptor agonists in fresh meat product (claimed). ADVANTAGE - The determination of beta -2 receptor agonists in fresh meat product can be economically carried out using the multi-walled carbon nanotube functionalized graphene composite material. DETAILED DESCRIPTION - A multi-walled carbon nanotube functionalized graphene composite material is prepared by mixing multi-walled carbon nanotubes and mixed acid in a volume ratio of 5:2, refluxing for 6 hours, centrifuging, washing solid twice with distilled water and vacuum-drying to obtain purified multi-walled carbon nanotubes, preparing graphene oxide by mixing 10 mL concentrated sulfuric acid, 4-5 g graphite powder, 2-2.5 g sodium nitrate, 92 mL water and potassium permanganate at room temperature in ice bath, adding hydrogen peroxide, filtering, washing filter cake twice with distilled water and vacuum-drying, preparing 0.1 mg/mL graphene oxide dispersion by diluting graphene oxide, adjusting pH of graphene oxide dispersion to 10-11 using 0.5 mol/L sodium hydroxide solution, then adding 35 mu L hydrazine hydrate and 350 mu L ammonia, reacting at 90 degrees C for 24 hours, centrifuging, washing and vacuum-drying to obtain reduced graphene oxide, preparing reduced graphene oxide dispersion by dissolving 1 mg redox graphene oxide in 1 mL distilled water, adding 10 mL sodium 1,3,6,8-pyrenetetrasulfonate solution to reduced graphene oxide dispersion, stirring at 65-75 degrees C for 4 hours, centrifuging and vacuum-drying to obtain functionalized graphene, preparing 0.5 mg/mL graphene suspension using functionalized graphene, mixing the graphene suspension with 3 mmol/L sodium 1,3,6,8-pyrenetetrasulfonate solution to obtain reaction solution, preparing 0.5 mg/mL multi-walled carbon nanotube dispersion by mixing water and purified multi-walled carbon nanotubes, mixing reaction solution and multi-walled carbon nanotube dispersion in a ratio of 1:1 at 65-75 degrees C for 5-6 hours, centrifuging, washing and vacuum-drying at 70-80 degrees C. The mixed acid comprises concentrated sulfuric acid and nitric acid in a volume ratio of 3:1. An INDEPENDENT CLAIM is included for usage method of multi-walled carbon nanotube functionalized graphene composite material which involves mixing methanol and 2 vol.% formic acid in a ratio of 1:9 to obtain formic acid aqueous solution, weighing 5 g fresh meat or meat product, extracting using 0.5 mL formic acid aqueous solution, regulating pH value of extract to 3, then adding 5 mg multi-walled carbon nanotube functionalized graphene composite material, extracting and carrying out liquid chromatography-mass spectrometry/mass spectrometry analysis.