▎ 摘　　要

NOVELTY - Immobilized enzyme reactor comprises stainless steel column tube at one end by way of vacuum suction alternately charged amino polymeric microspheres and graphene oxide from other side. Graphene oxide layers are assembled by way of adsorption on amino polymer microspheres to form composite matrix material. Then continuously fed into protease solution passing through electrostatic adsorption enzyme immobilized on matrix material to obtain immobilized enzyme reactor. USE - Immobilized enzyme reactor used in the field of proteomics or enzyme-catalyzed reaction, catalytic processes to achieve the reaction or enzymatic proteins (claimed). ADVANTAGE - Immobilized enzyme reactor has simple preparation, high capacity of immobilized enzymes and low back pressure.