▎ 摘　　要

NOVELTY - Preparation of nitrogen doped three-dimensional graphene supported carbon coated copper nanosubstrate material comprises dissolving trihydrate copper nitrate, glucose and sodium chloride in deionized water, stirring magnetically, adding urea containing nitrogen; freezing in refrigerator, placing in freeze drying machine, dehydrating, grinding, sieving and mixing to get powder precursor; placing in corundum ark under atmospheric condition, heating and cooling; and filtering with deionized water to remove sodium chloride and vacuum drying. USE - Method for preparing nitrogen doped three-dimensional graphene supported carbon coated copper nanosubstrate material used for coating substrate assembly (claimed). ADVANTAGE - The method is simple. The material enhances Raman spectroscopy with detection limit up to 1x 10-6 mol/L, good stability and excellent detection results. DETAILED DESCRIPTION - Preparation of nitrogen doped three-dimensional graphene supported carbon coated copper nanosubstrate material comprises dissolving trihydrate copper nitrate, glucose and sodium chloride in deionized water, where ratio of glucose and trihydrate copper nitrate is 2-5:1, ratio of sodium chloride and trihydrate copper nitrate is 100-200:1 and ratio of sodium chloride and water is 1:3-10, stirring magnetically, adding 10-100 times urea containing nitrogen to get homogeneous transparent precursor solution; freezing in refrigerator for 12-24 hours, placing in freeze drying machine at - 43 degrees C with less than 10 MPa vacuum degree for 24-48 hours, dehydrating, grinding, sieving and mixing to get powder precursor; placing in corundum ark under atmospheric condition, heating to 650-750 degrees C for 1-3 hours and cooling; and filtering with deionized water to remove sodium chloride and vacuum drying at 80 degrees C. An INDEPENDENT CLAIM is included for coating substrate assembly comprising placing nitrogen doped three-dimensional graphene supported carbon coated copper nanosubstrate material in container, adding ethanol in mass ratio of 1:80, ultrasonic dispersing, adding Pinus massoniana oil alcohol in mass ratio of 1:100, mixing magnetically, adding 10 wt.% ethyl cellulose ethanol solution in mass ratio of 1:10-20, ultrasonic dispersing until completely dissolve and evaporating to dryness at 60-80 degrees C in water bath to remove ethanol to get mixed slurry; placing layer of adhesive in both sides of pretreated glass slide, coating with mixed slurry using glass rod and heating at 90-130 degrees C for 5-15 minutes; and calcining in tube furnace under reduced atmosphere at 300-400 degrees C for 1-2 hours.