• 专利标题:   Mass-electrometry chip comprises array of transistors, each comprising of channel between electrodes with gate contact to modulate electronic response of channel and electrophoresis or focusing gel having gel-separated proteins, where chip is configured so that array provides digital signal readout.
  • 专利号:   WO2022178039-A1
  • 发明人:   ARAN K, CONBOY I M, CONBOY M J, KANE A A
  • 专利权人:   UNIV CALIFORNIA, KECK GRADUATE INST
  • 国际专利分类:   G01N027/403, G01N027/414, G01N027/447, G01N033/543, H01L029/66
  • 专利详细信息:   WO2022178039-A1 25 Aug 2022 G01N-027/414 202280 Pages: 26 English
  • 申请详细信息:   WO2022178039-A1 WOUS016671 16 Feb 2022
  • 优先权号:   US150075P

▎ 摘  要

NOVELTY - Mass-electrometry chip comprises an array of transistors, each comprising of a channel between two electrodes with a gate contact to modulate the electronic response of the channel, and an electrophoresis or focusing gel comprising gel-separated proteins, where the chip is configured so that the array provides a digital signal readout for fast multi-characteristic profiling and/or label-free identification of the proteins. The chip comprises liquid gated field effect transistors in a facile and miniaturized electrophoresis system configured to identify and measure the levels of proteins, based on their molecular weight and charge that are with molecular precision, digitally and in real-time. The gel is configured with distinct isoelectric focusing and gel electrophoresis areas, and the gel overlays the array, where the chip is configured to provide direct digital label-free identification, characterization and quantification of the proteins and real-time instantaneous fine resolution. USE - Mass-electrometry chip. ADVANTAGE - The mass-electrometry chip reduces the assay time and required starting sample, improves the accuracy and dynamic range of detection. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for using the chip.