• 专利标题:   DNA sequencing device comprises a silicon nitride film, a silicon-based substrate, the under layer contact electrode, and the bottom portion of contact electrode covered with a graphene microstrip.
  • 专利号:   CN105820947-A, CN105820947-B
  • 发明人:   DENG T, HOU J, LIU Y
  • 专利权人:   UNIV BEIJING JIAOTONG, UNIV BEIJING JIAOTONG
  • 国际专利分类:   C12M001/34, C12Q001/68, C12Q001/6869
  • 专利详细信息:   CN105820947-A 03 Aug 2016 C12M-001/34 201676 Pages: 10 Chinese
  • 申请详细信息:   CN105820947-A CN10319973 13 May 2016
  • 优先权号:   CN10319973

▎ 摘  要

NOVELTY - DNA sequencing device comprises a silicon nitride film is set on the silicon-based substrate. The under layer contact electrode is arranged above the silicon nitride film. The bottom portion of contact electrode is covered with a graphene microstrip. The hexagonal boron nitride microstrip is arranged on graphene microstrip. The top layer of graphene microstrip is arranged on hexagonal boron nitride microstrip. The graphene microstrip, hexagonal boron nitride microstrip and top graphene microstrip is set with graphene-hexagonal boron nitride-graphene heterojunction. USE - Used as DNA sequencing device. DETAILED DESCRIPTION - DNA sequencing device comprises a silicon nitride film is set on the silicon-based substrate. The under layer contact electrode is arranged above the silicon nitride film. The bottom portion of contact electrode is covered with a graphene microstrip. The hexagonal boron nitride microstrip is arranged on graphene microstrip. The top layer of graphene microstrip is arranged on hexagonal boron nitride microstrip. The graphene microstrip, hexagonal boron nitride microstrip and top graphene microstrip is set with graphene-hexagonal boron nitride-graphene heterojunction. The graphene-hexagonal boron nitride-graphene is etched at center of graphene hexagonal boron nitride-graphene nanopore. An INDEPENDENT CLAIM is also included for sequencing DNA using DNA sequencing device comprising adding single-stranded DNA molecules to electrolyte solution in lower portion of sequencing reaction chamber, driving by electrostatic field, adding single-stranded DNA molecules into linear graphene-hexagonal boron nitride-graphene nanopores, transferring into upper part of sequencing reaction chamber, transferring single-stranded DNA molecules through graphene-hexagonal boron nitride-graphene nano pores using a vertical weak current measurement device in process of transit time (t), where the time interval Delta t1 in which longitudinal ion current occludes, measuring the size of ionic current IB, transferring single-stranded DNA molecules to transverse weak tunneling current measurement device through top portion of graphene microstrip at a time interval of Delta t2 in which lateral tunneling current changes, carrying out quantitative measurement of horizontal tunneling current size on the IT horizontal carries, measuring longitudinal weak tunneling current using measurement device at a time interval Delta t3 at which the tunneling current changes using top and bottom portion of graphene, measuring longitudinal tunneling current in size IT-vertical, calculating the measured data of three groups by using a formula DNA molecule sequence = f (t, Delta t1, IB, Delta t2, IT-horizontal, Delta t3, IT-vertical), to obtain sequence information of DNA molecule. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic representation of the DNA sequencing device.