▎ 摘　　要

NOVELTY - Artificial cochlear electrode comprises platinum electrode as iridium and the conductive hydrogel loaded with neural stem cells and nerve growth factor with electrode coating by nano-composite cell three-dimensional load and ultraviolet curing process. The conductive hydrogel is Gelatin methylpropyl acrylate/graphene oxide-polyaniline (GelMA/GO-PAni) hydrogel. The electrode uses GelMA to realize neural stem cell transplantation which is good for neural stem cell survival and reducing stem cell cochlear diffusion. The combined nerve growth factor promotes neural stem cell differentiation and survival of residual auditory nerve using conductive GO-PAni nanometer filler to reduce electrode resistance and promoting nerve cell growth and differentiation. USE - Artificial cochlear electrode with nerve stem cell transplantation and signal conduction enhancement function. ADVANTAGE - The electrode increases the number of auditory nerves, reduces electrode resistance, and 0improves the hearing experience after cochlear implantation. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for A method for preparing artificial cochlear electrode with nerve stem cell transplantation and signal transduction enhancement function, which involves: (a) preparing a certain concentration of GO dispersion under the condition of high speed stirring, sequentially phytic acid aniline monomer, adding a raw material needing strong stirring, vortexing ultrasonic triple dispersion process for dispersion, dropping initiator solution under vigorous stirring condition free radical at normal temperature aggregate 4-8 hours, dialyzing drying to obtain GO-PAni conductive filler, dispersing in the deionized water to obtain dispersion; (b) dissolving the GelMA in the photo-initiator phosphate buffered saline (PBS) solution under the dark condition 60 degrees centigrade water bath, cooling the solution to room temperature, adding a certain amount of GO-PAni conductive filler dispersion, vortexing ultrasonic double dispersion process to make it mixed uniformly to obtain GelMA GO-PAni pre-solution; (c) adding nerve growth factor in GelMA GO-PAni solution in the dark condition vortex ultrasonic double dispersing process fully mixing uniformly centrifuging and defoaming; (d) adding GelMA GO-PAni pre-solution containing nerve growth factor under the dark condition in the neural stem cell deposition, fully mixing to obtain cell suspension, fixing the electrode substrate of the artificial cochlea in the mold under the dark condition, quickly transferring the cell suspension to the mold the 350-410nm wavelength light source 20-40mW/cm2irradiated for 15-40s demolding to obtain the artificial cochlear electrode.